Topic · Cell Biology · Biochemistry, Genetics and Molecular Biology
OpenAlex topic; a paper is listed when OpenAlex scores it at least 0.5 for this topic.
127 papers
Shuo Han, Boxuan Simen Zhao, Samuel A. Myers…·Alice Y. Ting
This study maps RNA-protein interactions in living cells using MS2 and Cas13-based APEX2 targeting, identifying novel interactions like hTR and ALKBH5.
Tomoya Niinae, Koshi Imami, Naoyuki Sugiyama, Yasushi Ishihama
This study identifies endogenous kinase substrates using proximity labeling and phosphoproteomics, finding 33 CK2 and 52 PKA substrates and linking mutations to altered phosphorylation.
Xianzhi Lin, Marcos A. Fonseca, Rosario I. Corona, Kate Lawrenson
This paper introduces RPL, a method to identify RNA-proximal proteins in human cells using CRISPR-Cas13b and APEX2, revealing new insights into RNA interactions and subcellular localization.
Leslie M. Stevens, Yuan Zhang, Yuri Volnov…·David S. Stein
The paper describes a modified BirA protein that enables biotinylation of secreted proteins in Drosophila, allowing their isolation from ovaries and embryos.
Alejandro Padrón, Shintaro Iwasaki, Nicholas T. Ingolia
The paper introduces APEX-Seq, a proximity labeling technique to map RNA localization and interactions, revealing insights into translation initiation complexes and stress granule composition.
Xin D. Gao, Li‐Chun Tu, Aamir Mir…·Erik J. Sontheimer
C-BERST uses dCas9-APEX2 to map proteomes near genomic loci, identifying telomere and centromere-associated proteins.
Xiaonan Liu, Kari Salokas, Fitsum Tamene…·Markku T Varjosalo
The paper develops an integrated approach combining AP-MS and BioID to map protein interactions and molecular contexts across the human proteome.