Shintaro Iwasaki

The paper introduces a method to quantify tRNA in low-input samples using RNA-dependent RNA amplification with T7 polymerase, showing reproducible and quantitative results.

The paper identifies neuronal stop-codon readthrough in Drosophila, showing it produces C-terminal extensions that alter protein localization.

The paper investigates mitochondrial translation termination, recycling, and quality control mechanisms using Ribo-Seq and Disome-Seq, revealing roles of release factors and rescue mechanisms.

The paper uses optogenetic stimulation and Ribo-seq in Drosophila to show that neuronal activity triggers two phases of translation: one for synaptic factors and another for ribosomal proteins, regulated by TOP motifs and La-related protein.

This study identifies NudC as a novel regulator of ribosome biogenesis and homeostasis in Drosophila polyploid cells, independent of its known role in dynein regulation.

The paper identifies ribosome stalling as a mechanism that enhances chloroplast targeting of nuclear-encoded proteins in Arabidopsis thaliana.

The paper identifies translational regulation of GAD1 in the mouse SCN using Ribo-seq and RNA-seq, showing light-induced ribosome binding without mRNA changes.

The paper shows how mimiviruses create a subcellular environment to overcome codon-tRNA mismatch by localizing viral mRNAs and proteins at the periphery of a viral factory.

This study investigates the role of coilin in mice by generating coilin mutants, revealing its involvement in snRNA modifications and the formation of novel condensates in spermatids.

The paper develops a semi-automated Ribo-Seq and Disome-Seq platform to analyze translation status across many samples, identifying factors regulating translation and revealing links between stop codon readthrough and mitochondrial translation deficiency in COVID-19.

APEX-Ribo-Seq maps local translation across organelles and identifies compartment-specific transcripts, revealing sequence features and dynamics of subcellular translation.

This study shows that eIF4G2 is essential for intestinal stem cell maintenance and differentiation by facilitating the translation of histone modifiers.

This study identifies EIF3D as a key regulator of primed pluripotency through selective translational control of signaling pathways and p53 regulators.

This study shows that eRF1 transiently binds to UUA sense codons, causing ribosome stalling and triggering quality control mechanisms in human cells.

The paper investigates the coordination of chloroplast and cytoplasmic protein synthesis during light/dark transitions in plants, revealing translational control in chloroplasts and mRNA abundance control in the cytoplasm.

The paper examines translation variation across yeast genetic backgrounds and identifies a post-transcriptional buffering signature that affects essential genes and low-transcribed genes.

This study investigates the effects of eIF5A hypusination inhibition on mitochondrial function and identifies MPV17L2 as a key regulator involved in mitochondrial integrity.

The paper shows that translational regulation, particularly through 5' leader uORFs, enhances cell-type distinction in the Drosophila nervous system by preferentially translating neuronal proteins in neurons.

This paper identifies pateamine A as a small-molecule compound that selectively represses mRNA translation by anchoring eIF4A and DDX3 to GNG motifs.

This study shows that Mettl1-dependent m7G tRNA modification is essential for spermatogenesis and fertility in Drosophila, as its loss leads to sterility and tRNA instability.

This study develops a high-resolution mitochondrial ribosome profiling technique to analyze translation dynamics and regulation in mammals.

The paper investigates how Znf598-mediated no-go decay (NGD) degrades mRNAs encoding C2H2-ZF domains during zebrafish development, preventing ribosome collisions and maintaining translational homeostasis.

This paper introduces methods to absolute quantify ribosome flux on transcripts, revealing ribosome allocation under stress, aging, and across cell types.

CRISPRδ uses dCas13 to specifically block translation without affecting mRNA stability, achieving high gene knockdown specificity in mammalian cells.

The paper shows that gravity and mechanical forces influence mitochondrial translation by affecting signaling pathways and malonylation of the translational machinery.

Thor-Ribo-Seq enables ribosome profiling on low-input samples using RNA-dependent RNA amplification to assess ribosome traversal with codon resolution.

The paper identifies a fungal mutation that allows survival on plants producing toxic rocaglates by altering eIF4A, a target of these compounds.

This study shows that GW182 is essential for miRNA-mediated gene silencing in Drosophila, affecting larval development despite some survival and repression capabilities.

The paper investigates paraspeckle formation in naked mole-rats, finding that nNEAT1_2 recruits different RNA binding proteins compared to mice.

This study identifies METTL18 as a histidine methyltransferase that modifies RPL3, slowing ribosome translocation on tyrosine codons to aid protein folding and prevent aggregation.