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CASPE is a lightweight platform for site-directed protein engineering that improves the identification of beneficial mutations for thermostability and pH tolerance.

This paper introduces a platform for efficient protein delivery using extracellular vesicles engineered with biogenesis-inspired features, achieving functional gene editing in vitro and in vivo.

This paper introduces an adjustable ankle-foot orthosis (OLAFO) that provides controlled offloading during gait, demonstrating its feasibility through biomechanical testing in an able-bodied participant.

This paper introduces a carbon cofeeding strategy to overcome stoichiometric limitations in redox-balance growth coupling, enabling growth-coupled selection for biosynthetic pathways in engineered E. coli.

This study uses micropillar compression and imaging to analyze mechanical properties of subchondral bone in osteoarthritis, finding increased strength in trabeculae but unchanged modulus, with mineralization as a key determinant.

This paper develops a low-input, ultrasensitive diagnostic platform for detecting Avocado Sunblotch Viroid using RPA-CRISPR and nanopore-array fluorescence readout, achieving a detection limit of 1.68 copies/L.

This study investigates thiol-ene crosslinking dynamics in hyaluronic acid hydrogels to optimize network properties for in vitro tissue modeling.

The paper uses a multi-organ microphysiological system to show that liver-derived cyclophosphamide metabolites cause neurotoxicity in connected CNS organoids.

The paper introduces a new chemical method, SAPLabTech, to create self-assembling artificial proteins with functional diversity by using supramolecular complexes for site-specific conjugation.

This paper introduces a feature-preserving conditional GAN for multi-site MRI harmonization, showing improved performance over existing methods.

This paper engineers the PirC-PGAM switch in cyanobacteria to redirect carbon flux toward desired products like sucrose and succinate, demonstrating a tunable control point for metabolic engineering.

This study develops multidimensional in vitro models to investigate anti-estrogen resistance and dormancy in invasive lobular carcinoma, identifying p27Kip1 signaling as central to dormant phenotype.

This study demonstrates that GROQ-seq provides reproducible, scalable measurements of protein function across different facilities.

The paper reports a method to reprogram primary human hepatocytes into proliferative pre-cHep cells with high expansion potential and liver repopulation capacity, offering an alternative to PHHs for drug testing and disease modeling.

This paper develops a CRISPR-based assay for rapid detection of cefixime resistance in Neisseria gonorrhoeae by analyzing penA gene mosaicism.

An acellular spleen perfusion platform enables serial multiomic sampling to identify steroid-responsive transcriptional programs, validate SOCS3 protein induction, and nominate a non-transcriptional immunosuppressive axis.

This paper introduces an in vitro platform with a self-sustaining oxygen gradient to better model intestinal barrier function and improve drug absorption predictions.

This study shows that estrogen-mimetic compounds in cell culture media affect human mesenchymal stromal cells differently in males and females.

This paper develops a composite biomaterial combining polyelectrolyte complex micelles with hyaluronic acid gels to deliver miR-92a inhibitors, enhancing angiogenesis and accelerating wound healing in diabetic mice.

This paper develops COX-2-targeted nanobodies for enhanced endoscopic imaging of colorectal adenomas, showing tumor-specific accumulation in vivo.