Discover, rate, and discuss biology preprints.

The paper identifies ZNF654 and JMJD6 as novel factors defining TAD boundaries through in silico screens and experimental validation.

RNA BaseCode is a synthetic long-read RNA sequencing method that achieves short-read accuracy and improves full-length transcript reconstruction compared to existing long-read methods.

This study compares commercial single-cell RNA sequencing platforms based on performance metrics and practical considerations.

This study shows that overexpressing an Alu transposon in human fibroblasts causes widespread disruptions in aging-related pathways across transcriptome, proteome, and secretome.

This study uses whole-Y sequencing to identify and date Cohen lineages, revealing nine major branches and their geographic dispersal across the Jewish Diaspora.

The paper presents a lineage-wide evolutionary analysis of 3D genome architecture and organization across brown algae, revealing conserved chromosomal features, lineage-specific rearrangements, and long-term co-evolution of centromeres and retrotransposons.

panHiTE detects population-specific transposable elements in large-scale plant genomes, improving sensitivity and precision while reducing redundancy and enabling TE-gene associations.

The paper investigates co-speciation and genome reduction in Wolbachia of Neotropical Drosophila, finding congruent topologies but low mutation rates and unexpected genome size increase in an obligate strain.

This study evaluates over 3,000 congenital heart disease-risk variants using SNP Bind-n-Seq and MPRA to identify variants with allelic TF binding and regulatory activity.

RoCK and ROI improves single-cell transcriptomics by targeting specific transcripts and regions, enhancing detection of critical biological features.

The paper develops a minimal model of cohesin loop extrusion based on biochemical and biophysical data, showing how regulatory protein dynamics influence genome folding.

The paper identifies alveolar macrophage-specific oncostatin M as a driver of pulmonary fibrosis in PCV2d infection and shows targeting it reduces fibroblast activation.

This paper compares two human genetic interaction maps generated using radiation hybrids and CRISPRi, showing they share some interactions but differ in gene pair specificity and GWAS network overlap.

The paper identifies a new SET1B complex and shows SET1/MLL complexes control transcription independently of H3K4me3 by counteracting promoter-proximal termination and supporting transcription burst size.

The paper applies spatial statistical models to spatialomics data to reveal detailed morphogenic field interactions in tissues.

The paper performs genome-wide association analysis to identify genetic loci and candidate genes associated with uncapping behavior in Varroa-resistant honeybees, highlighting a polygenic basis for this trait.

This study identifies 5'UTR exons containing uORFs as targets for splice-switching to increase protein translation in haploinsufficient disease genes.

The paper investigates how centromere clustering states in Cryptococcus neoformans influence 3D genome architecture during the cell cycle, revealing unique organizational principles.

This study identifies a major QTL on chromosome 21 in coho salmon linked to SRS resistance, implicating two candidate genes, hint3 and a lncRNA, through SNP analysis and expression correlation.