A. Bremer

Design of intrinsically disordered protein variants with diverse structural properties

This paper introduces a physics-based algorithm to design intrinsically disordered proteins with specified structural properties, demonstrating high accuracy in predicting compaction and phase separation behavior.

Crowder titrations enable the quantification of driving forces for macromolecular phase separation

This paper demonstrates how crowder titrations can quantify intrinsic driving forces for macromolecular phase separation by measuring saturation concentrations and second virial coefficients.

Sequence-specific interactions determine viscoelasticity and aging dynamics of protein condensates

This study investigates how sequence-specific interactions influence the viscoelastic properties and aging dynamics of protein condensates formed by prion-like low-complexity domains.

Phase Separation in Mixtures of Prion-Like Low Complexity Domains is Driven by the Interplay of Homotypic and Heterotypic Interactions

This paper investigates phase separation of mixtures of prion-like low complexity domains using simulations and experiments, finding that homotypic and heterotypic interactions drive condensate formation.

A complete digital karyotype of the B-cell leukemia REH cell line resolved by long-read sequencing

This paper creates a complete digital karyotype of the REH B-cell leukemia cell line using long-read and short-read sequencing technologies, identifying structural variants and fusion genes.

Quantifying coexistence concentrations in multi-component phase-separating systems using analytical HPLC

This paper develops an analytical HPLC method to quantify coexisting phase concentrations in multi-component phase-separating systems, enabling reconstruction of coexistence curves.

Condensates of disordered proteins have small-world network structures and interfaces defined by expanded conformations

This study shows that disordered protein condensates form small-world networks with interface-specific conformational expansions and orientations.

Deciphering how naturally occurring sequence features impact the phase behaviors of disordered prion-like domains

The paper identifies how specific amino acid compositions influence phase separation in prion-like domains and develops a model to simulate these behaviors.

Microfluidic characterization of liquid-liquid phase separation

The paper introduces a microfluidic chip to measure phase separation of biomolecules with reduced sample quantity and improved statistical accuracy.