M. Farag

Condenzymes: Biomolecular condensates with inherent catalytic activities

The paper discovers that intrinsically disordered proteins form condensates with catalytic activity through sequence-dependent electrochemical microenvironments, enabling hydrolysis reactions and gene circuit activation in cells.

Tunable metastability of condensates reconciles their dual roles in amyloid fibril formation

The paper shows that separable interactions in A1-LCD allow tunable metastability of condensates, which can suppress amyloid fibril formation even with ALS mutations.

Dominance Analysis: A formalism to uncover dominant energetic contributions to biomolecular condensate formation in multicomponent systems

This paper introduces a formalism to identify dominant energetic contributions to biomolecular condensate formation in multicomponent systems by analyzing phase separation data.

Sequence-specific interactions determine viscoelasticity and aging dynamics of protein condensates

This study investigates how sequence-specific interactions influence the viscoelastic properties and aging dynamics of protein condensates formed by prion-like low-complexity domains.

FIREBALL: A tool to fit protein phase diagrams based on mean-field theories for polymer solutions

FIREBALL fits protein phase diagrams using mean-field theories to analyze phase separation parameters from experimental or computed data.

Phase Separation in Mixtures of Prion-Like Low Complexity Domains is Driven by the Interplay of Homotypic and Heterotypic Interactions

This paper investigates phase separation of mixtures of prion-like low complexity domains using simulations and experiments, finding that homotypic and heterotypic interactions drive condensate formation.

Single fluorogen imaging reveals distinct environmental and structural features of biomolecular condensates

This study uses single-fluorogen imaging to reveal that biomolecular condensates have hydrophobic internal environments and spatially inhomogeneous molecular organization.

Adsorption of RNA to interfaces of biomolecular condensates enables wetting transitions

This study shows that pyrimidine-rich RNAs act as adsorbents enabling wetting transitions in condensates formed by purine-rich RNAs, revealing orientation differences between scaffolds and adsorbents.

Uncovering molecular grammars of intrinsically disordered regions that organize nucleolar fibrillar centers

The paper identifies molecular grammars in intrinsically disordered regions of nucleolar proteins that drive phase separation to form nucleolar substructures.

Quantifying coexistence concentrations in multi-component phase-separating systems using analytical HPLC

This paper develops an analytical HPLC method to quantify coexisting phase concentrations in multi-component phase-separating systems, enabling reconstruction of coexistence curves.

Condensates of disordered proteins have small-world network structures and interfaces defined by expanded conformations

This study shows that disordered protein condensates form small-world networks with interface-specific conformational expansions and orientations.

Deciphering how naturally occurring sequence features impact the phase behaviors of disordered prion-like domains

The paper identifies how specific amino acid compositions influence phase separation in prion-like domains and develops a model to simulate these behaviors.