Author

A. E. Posey

Listed by name: these papers carry no ORCID for this author, so different people with the same name may be mixed together.

Biology Disciplines

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Biophysics· 8 Biochemistry· 1 Cell Biology· 1

Signature Assays, Organisms & Methods

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Approach:experimental assay· 6 Research Focus:cell signaling & trafficking· 5 Assay:proteomics· 5 Approach:statistical model· 5 Research Focus:protein folding & dynamics· 4 Research Focus:cell type annotation· 2 Research Focus:chromatin & epigenetic regulation· 2 Approach:biophysical modeling· 1

Frequent Co-Authors

R. V. Pappu· 8 shared A. Bremer· 2 shared A. S. Gladfelter· 2 shared I. Seim· 2 shared J. M. Lalmansingh· 2 shared K. M. Ruff· 2 shared M. Farag· 2 shared M. J. Fossat· 2 shared

Activity by Year

2019-2023 · peak 4/yr

Co-Author Collaboration Network

10 top collaborators · 22 mutual co-author links

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8 shared papersBiophysics

R. V. Pappu

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10 papers

Recent·Most cited

The variable domain from the mitochondrial fission mechanoenzyme Drp1 promotes liquid-liquid phase separation

A. E. Posey, M. Bagheri, K. A. Ross, E. N. Lanum, M. A. Khan, C. M. Jennings, M. C. Harwig, N. W. Kennedy, V. J. Hilser, J. L. Harden, R. B. Hill

The paper shows the Drp1 variable domain undergoes liquid-liquid phase separation under crowding conditions, which may regulate mitochondrial fission.

bioRxiv · May 29, 2023Biophysics

Sequence-specific interactions determine viscoelasticity and aging dynamics of protein condensates

I. Alshareedah, W. M. Borcherds, S. R. Cohen, A. Singh, A. E. Posey, M. Farag, A. Bremer, G. W. Strout, D. T. Tomares, R. V. Pappu, T. Mittag, P. R. Banerjee

This study investigates how sequence-specific interactions influence the viscoelastic properties and aging dynamics of protein condensates formed by prion-like low-complexity domains.

bioRxiv · Apr 6, 2023Biophysics

Uncovering the contributions of charge regulation to the stability of single alpha helices

M. J. Fossat, A. E. Posey, R. V. Pappu

The paper investigates how charge regulation affects the stability of single alpha helices, finding that uncharged glutamate residues and conformational buffering contribute to pH insensitivity.

ChemPhysChem · Jan 4, 2023Peer-reviewedBiophysics

Dynamical control enables the formation of demixed biomolecular condensates

A. Z. Lin, K. M. Ruff, A. P. Jalihal, F. Dar, M. R. King, J. M. Lalmansingh, A. E. Posey, I. Seim, A. S. Gladfelter, R. V. Pappu

This study identifies dynamical control as a mechanism enabling the formation of demixed biomolecular condensates by showing that asynchronous molecular production is required for their coexistence.

bioRxiv · Jan 4, 2023Biophysics

The soluble N-termini of mechanosensitive ion channels MSL8, MSL9, and MSL10 are environmentally sensitive intrinsically disordered regions with distinct biophysical characteristics

A. J. Flynn, K. Miller, J. M. Codjoe, M. R. King, A. E. Posey, E. S. Haswell

This study identifies and characterizes intrinsic disorder in the N-terminal domains of MSL8, MSL9, and MSL10, showing distinct biophysical properties and environmental responsiveness.

bioRxiv · Oct 14, 2022 · PreprintBiochemistry

Quantifying coexistence concentrations in multi-component phase-separating systems using analytical HPLC

A. Bremer, A. E. Posey, M. B. Borgia, W. M. Borcherds, M. Farag, R. V. Pappu, T. Mittag

This paper develops an analytical HPLC method to quantify coexisting phase concentrations in multi-component phase-separating systems, enabling reconstruction of coexistence curves.

bioRxiv · Sep 11, 2022Biophysics

Quantifying charge state heterogeneity for proteins with multiple ionizable residues

M. J. Fossat, A. E. Posey, R. V. Pappu

This paper introduces a q-canonical ensemble framework to analyze potentiometric titration data and determine mesostate pKa values and populations for proteins with multiple ionizable residues.

bioRxiv · Aug 31, 2021Biophysics

Dilute phase oligomerization can oppose phase separation and modulate material properties of a ribonucleoprotein condensate

I. Seim, A. E. Posey, W. T. Snead, B. M. Stormo, D. Klotsa, R. V. Pappu, A. S. Gladfelter

This study shows that a specific sequence motif in the Whi3 protein promotes dilute phase oligomerization, which opposes phase separation and alters the composition and material properties of ribonucleoprotein condensates.

bioRxiv · Apr 19, 2021Biophysics

Mechanistic inferences from analysis of measurements of protein phase transitions in live cells

A. E. Posey, K. M. Ruff, J. M. Lalmansingh, T. S. Kandola, J. J. Lange, R. Halfmann, R. V. Pappu

This paper develops a supervised method to classify phase transitions in live cells using DAmFRET data, revealing sequence features that drive prion-like behavior and enabling quantitative analysis of nucleation probabilities.

bioRxiv · Nov 4, 2020Biophysics

Arginine-enriched mixed-charge domains provide cohesion for nuclear speckle condensation

J. A. Greig, T. A. Nguyen, M. Lee, A. S. Holehouse, A. E. Posey, R. V. Pappu, G. J. Jedd

The paper identifies sequence features of mixed-charge domains that govern nuclear speckle condensation and function in mRNA processing.

bioRxiv · Sep 16, 2019Cell Biology