Conformational transitions of the mitotic adaptor Spindly underlie its interaction with Dynein and Dynactin
E. d'Amico, M. Ud Din Ahmad, V. Cmentowski, M. Girbig, F. Mueller, S. Wohlgemuth, A. Brockmeyer, S. Maffini, P. Janning, I. R. Vetter, A. P. Carter, A. Perrakis, A. Musacchio
The paper shows Spindly adopts a closed conformation that blocks Dynein-Dynactin binding, and mutations induce an open state that enables interaction, with kinetochores promoting Spindly activation.
bioRxiv · Feb 2, 2022Biochemistry