S. Wohlgemuth

Bisphenol TMC disturbs mitochondrial activity and biogenesis, reducing lifespan and healthspan in the nematode Caenorhabditis elegans

This study shows that BPTMC exposure in C. elegans causes mitochondrial dysfunction, reduced lifespan, and healthspan, raising concerns about its safety as a BPA substitute.

A mechanism that integrates microtubule motors of opposite polarity at the kinetochore corona

The paper identifies CENP-E's role in regulating DD kinetochore loading through the corona scaffold, ensuring coordinated bidirectional transport.

Conformational transitions of the mitotic adaptor Spindly underlie its interaction with Dynein and Dynactin

The paper shows Spindly adopts a closed conformation that blocks Dynein-Dynactin binding, and mutations induce an open state that enables interaction, with kinetochores promoting Spindly activation.

Structure of the RZZ complex and molecular basis of Spindly-driven corona assembly at human kinetochores

The paper reports the structure of the RZZ complex and demonstrates the molecular mechanism of corona assembly at human kinetochores using cryo-EM and in vitro assays.

Reconstitution and use of highly active human CDK1:Cyclin-B:CKS1 complexes

The paper develops and compares protocols to produce and use highly active CDK1:Cyclin-B and CDK1:Cyclin-B:CKS1 complexes for mitotic protein machinery studies.

Electroporated recombinant proteins as tools for in vivo functional complementation, imaging, and chemical biology

This study demonstrates that electroporation can deliver recombinant proteins into human cells, maintaining their localization, function, and interactions, making it a versatile tool for chemical and synthetic biology.