A. Perrakis

Structural plasticity of 2A proteins in the Parechovirus family

The paper investigates structural plasticity in Parechovirus 2A proteins, revealing structural rearrangements that affect their oligomeric state and potential function in viral RNA replication.

Caskin2 is a novel talin and Abi1-binding protein that promotes cell motility

This study identifies Caskin2 as a novel talin-binding protein that enhances cell motility through interactions with Abi1 and the WAVE Regulatory Complex.

Distant sequence regions of JBP1 contribute to J-DNA binding

The paper investigates how JBP1's JDBD binds to J-DNA using crystal structures, MD simulations, and mutagenesis, revealing the role of the N-terminus in DNA binding.

Autotaxin facilitates selective LPA receptor signaling

This study shows that ATX inhibitors targeting the ATX tunnel selectively block LPA signaling via P2Y receptors, with implications for pulmonary fibrosis treatment.

Conformational transitions of the mitotic adaptor Spindly underlie its interaction with Dynein and Dynactin

The paper shows Spindly adopts a closed conformation that blocks Dynein-Dynactin binding, and mutations induce an open state that enables interaction, with kinetochores promoting Spindly activation.

A tomato prolyl-4-hydroxylase causes relocation of abscission zone and alters abscission kinetics

This study shows that altering tomato SlP4H3 expression shifts the abscission zone position and modifies ethylene-induced abscission timing depending on the developmental stage.

The MPS1 kinase NTE region has helical propensity and preferred conformations towards the TPR domain

This study investigates the conformational dynamics of the MPS1 kinase NTE region relative to the TPR domain using paramagnetic NMR and Rosetta, revealing helical propensity and convex surface interactions.

A global Ramachandran score identifies protein structures with unlikely stereochemistry

The paper introduces a re-implemented Ramachandran Z-score and its uncertainty estimation for protein structure validation, advocating its inclusion in structural publications and validation reports.

Autotaxin impedes anti-tumor immunity by suppressing chemotaxis and tumor infiltration of CD8+ T cells

This study shows that ATX secreted by melanoma cells repels CD8+ T cells, reducing their infiltration into tumors and impairing anti-tumor immunity.

The domain architecture of JBP1 suggests synergy between J-base DNA binding and thymidine hydroxylase activity

This paper investigates the domain architecture of JBP1, showing that the JDBD domain enhances thymidine hydroxylation activity by facilitating J-DNA recognition.

Interactions between N-terminal modules in MPS1 enable spindle checkpoint silencing

This study identifies an NTE-TPR interaction in MPS1 essential for SAC silencing by regulating MPS1 localization and delocalization from kinetochores.

Lysophosphatidic acid produced by Autotaxin acts as an allosteric modulator of its catalytic efficiency

The paper investigates how lysophosphatidic acid (LPA), produced by autotaxin (ATX), modulates its own catalytic efficiency through allosteric regulation.

Characterisation and structure determination of a llama-derived nanobody targeting the J-base binding protein 1

The paper characterizes a llama-derived nanobody targeting JBP1, showing high affinity and solving its crystal structure to 1.64 Å resolution, with insights into CDR flexibility.

Dynamic Kinetochore Size Regulation Promotes Microtubule Capture And Chromosome Biorientation In Mitosis

The paper shows that Spindly-based dynamic regulation of kinetochore size ensures proper microtubule capture and chromosome segregation by controlling corona expansion and shedding.

CLIC4 is regulated by RhoA-mDia2 signaling through Profilin-1 binding to modulate filopodia length

This study identifies RhoA-mDia2 signaling and Profilin-1 binding as regulators of CLIC4 translocation, which modulates filopodia length through actin dynamics.

Understanding inhibitor resistance in Mps1 kinase through novel biophysical assays and structures

The paper characterizes inhibitor resistance in Mps1 mutants using biophysical assays and crystal structures, showing that Cpd-5 is better tolerated than NMS-P715 in resistant mutants.

Homology-based hydrogen bond information improves crystallographic structures in the PDB

The paper improves low-resolution crystallographic structures by incorporating homology-derived hydrogen bond restraints.

Negative regulation of uPAR activity by a GPI-specific phospholipase C

This study identifies GDE3 as a GPI-specific phospholipase C that cleaves uPAR, reducing its proteolytic and non-proteolytic activities and altering cell behavior in breast cancer.