Cancer BiologybioRxiv
An in vitro tumorigenesis model based on live cell-generated oxygen and nutrient gradients
A. C. Gilmore, S. J. Flaherty, V. Somasundaram…·W. F. Heinz
This paper introduces a 2D live-cell chamber to model the tumor microenvironment, showing that breast cancer cells and macrophages generate hypoxic and nutrient gradients and exhibit spatially varying phenotypes.