Dub-seq: dual-barcoded shotgun expression library sequencing for high-throughput characterization of functional traits
V. K. Mutalik, P. S. Novichkov, M. N. Price…·A. P. Arkin
Dub-seq enables high-throughput overexpression screens in E. coli by combining shotgun expression libraries with DNA barcode sequencing to identify genes with gain-of-function phenotypes.