MicrobiologybioRxiv
CRISPRi screen for enhancing heterologous α-amylase yield in Bacillus subtilis
A. S. Geissler, A. O. Fehler, L. D. Poulsen…·J. Gorodkin
The paper uses CRISPRi to knock down non-coding RNA regions in Bacillus subtilis to identify those affecting α-amylase yield, finding some antisense RNAs increase or decrease yield.
DOI 10.1093/jimb/kuac028