Evolutionary BiologybioRxiv
Evolution of CRISPR-associated Endonucleases as Inferred from Resurrected Proteins
B. Alonso-Lerma, Y. Jabalera, M. Morin…·R. Perez-Jimenez
This study resurrects ancient CRISPR-Cas9 nucleases to show their greater flexibility in PAM and gRNA requirements and their evolution toward double-strand break activity.
DOI 10.1038/s41564-022-01265-y