Revealing the Oligomerization of Channelrhodopsin-2 in the Cell Membrane using Photo-Activated Localization Microscopy
E. Bestsennaia, I. Maslov, T. Balandin, A. Alekseev, A. Yudenko, A. A. Shamseye, D. Zabelskii, A. Baumann, C. Catapano, C. Karathanasis, V. Gordeliy, M. Heilemann, T. Gensch, V. Borshchevskiy
This study uses qPALM to show ChR2 predominantly forms dimers in the cell membrane, with disulfide bonds not essential for dimerization and partial monomerization observed at low concentrations.
bioRxiv · May 24, 2023 · PreprintBiophysics