M. Heilemann

Long-term single-molecule tracking in living cells using weak-affinity protein labeling

This paper introduces a labeling method for long-term single-molecule tracking in living cells and demonstrates its application to study EGFR dynamics.

Self-quenched fluorophore-DNA labels for super-resolution fluorescence microscopy

The paper introduces self-quenched fluorophore-DNA labels to reduce background signal in super-resolution microscopy, improving imaging performance in STED and SMLM.

Single-molecule imaging and molecular dynamics simulations reveal early activation of the MET receptor in situ

The paper uses single-molecule imaging and molecular dynamics simulations to study early MET receptor activation in situ, revealing structural dynamics and a refined dimer model.

The molecular architecture of the kidney slit diaphragm

The paper uses advanced imaging techniques to elucidate the molecular architecture of the kidney slit diaphragm, revealing a fishnet-like structure and interactions between key proteins.

Transertion and cell geometry organize the Escherichia coli nucleoid during rapid growth

This study shows that transertion and cell geometry influence the spatial organization of the E. coli nucleoid during growth and antibiotic treatment.

Revealing the Oligomerization of Channelrhodopsin-2 in the Cell Membrane using Photo-Activated Localization Microscopy

This study uses qPALM to show ChR2 predominantly forms dimers in the cell membrane, with disulfide bonds not essential for dimerization and partial monomerization observed at low concentrations.

The function of ER-phagy receptors is regulated through phosphorylation-dependent ubiquitination pathways

This study identifies CK2-dependent phosphorylation and ubiquitination as essential for FAM134B and FAM134C-driven ER-phagy activation during mTOR inhibition.

Biased activation of the receptor tyrosine kinase HER2

This study measures HER2 activation in live cells using single-molecule tracking and finds ligand-specific activation strength and temporal profiles.

DBlink: Dynamic localization microscopy in super spatiotemporal resolution via deep learning

DBlink improves super spatiotemporal resolution in SMLM by using deep learning to reconstruct dynamic processes from video data.

Exchangeable HaloTag Ligands (xHTLs) for multi-modal super-resolution fluorescence microscopy

The paper introduces exchangeable HaloTag ligands for multi-modal super-resolution fluorescence microscopy, enabling dual-color imaging techniques like PAINT and STED.

Dynamic in situ confinement triggers ligand-free neuropeptide receptor signaling

This study shows that dynamic in situ confinement of the Y2R receptor triggers ligand-independent signaling through cytosolic calcium increases, cell spreading, and migration.

Fast DNA-PAINT imaging using a deep neural network

The paper trains a neural network to accelerate DNA-PAINT imaging, reducing acquisition time to one minute while maintaining super-resolution capabilities.

DeepBacs: Bacterial image analysis using open-source deep learning approaches

DeepBacs uses open-source deep learning to analyze bacterial microscopy images, offering tools for segmentation, classification, and phenotyping with applications in antibiotic research.

A framework for evaluating the performance of SMLM cluster analysis algorithms

The paper evaluates SMLM cluster analysis algorithms using ARI and IoU metrics across simulated scenarios to establish a standard framework for future algorithm development.

KAHRP dynamically relocalizes to remodeled actin junctions and associates with knob spirals in P. falciparum-infected erythrocytes

This study uses super-resolution microscopy to show KAHRP relocalizes to remnant actin junctions and associates with spiral scaffolds in P. falciparum-infected erythrocytes.

Visualizing multi-protein patterns at the synapse of neuronal tissue with DNA-assisted single-molecule localization microscopy

This paper introduces a method to visualize multiple proteins at synapses using DNA-assisted single-molecule localization microscopy, enabling high-resolution imaging of neuronal tissue sections.

Serine-ubiquitination regulates Golgi morphology and the secretory pathway upon Legionella infection

This study shows that Legionella's SdeA effector causes Golgi disruption via serine ubiquitination of GRASP55 and GRASP65, affecting the secretory pathway and cytokine release.

Bleaching-independent, whole-cell, 3D and multi-color STED imaging with exchangeable fluorophores

The paper introduces a bleaching-independent STED microscopy method using exchangeable fluorophores, enabling long-term whole-cell 3D and multi-color imaging in live cells.

CD95 receptor activation by ligand-induced trimerization is independent of its partial pre-ligand assembly

This paper investigates how CD95 receptor activation occurs through ligand-induced trimerization, showing that receptor multimerization follows ligand binding while FADD recruitment is delayed, and that soluble CD95L is a poor inducer of apoptosis due to inefficient FADD recruitment.