Technical insights into fluorescence lifetime microscopy of mechanosensitive Flipper probes
C. Roffay, J. M. Garcia Arcos, P. Chapuis, J. Lopez-Andarias, F. Schneider, A. Colom, C. Tomba, I. Di Meglio, V. Dunsing, S. Matile, A. Roux, V. Mercier
The paper optimizes fluorescence lifetime imaging (FLIM) for mechanosensitive Flipper probes to measure membrane tension and long-range forces in cells, tissues, and organs.
bioRxiv · Sep 28, 2022 · PreprintBiophysics