J. Guck

Size-Dependent Mechanoadaptation Enables Migration with Oversized Parasitic Cargo

The paper investigates how cells adapt to transport oversized parasitic cargo by reprogramming mechanical forces and subcellular architecture during migration.

Cell viscosity influences hematogenous dissemination and metastatic extravasation of tumor cells

This study shows that tumor cell viscosity affects their ability to circulate and extravasate, influencing metastasis.

Optical characterization of molecular interaction strength in protein condensates

The paper introduces optical techniques to measure molecular interaction strength and physical properties of protein condensates, showing how sequence variations and environmental factors affect these properties.

Beyond comparison: Brillouin microscopy and AFM-based indentation reveal divergent insights into the mechanical profile of the murine retina

This study compares Brillouin microscopy and AFM-based indentation to assess mechanical properties of murine retinal tissues, finding divergent results and highlighting their complementary nature.

High-throughput viscoelastic characterization of cells in hyperbolic microchannels

This paper introduces hyperbolic microchannels for high-throughput viscoelastic measurement of cells and soft particles, showing accurate recovery of mechanical properties under controlled stress.

Estimation of the mass density of biological matter from refractive index measurements

This paper estimates mass density from refractive index measurements using a method that incorporates biochemical composition assumptions and uncertainty analysis, validated on biological samples.

Membrane to cortex attachment determines different mechanical phenotypes in LGR5+ and LGR5- colorectal cancer cells

This study identifies mechanical phenotype differences between LGR5+ and LGR5- colorectal cancer cells, linking them to membrane to cortex attachment proteins and metastatic potential.

Conserved nucleocytoplasmic density homeostasis drives cellular organization across eukaryotes

This study shows that the nuclear-to-cytoplasmic density ratio is conserved across eukaryotes and drives cellular organization, with implications for diseases like cancer and senescence.

Shear rheology of methyl cellulose based solutions for cell mechanical measurements at high shear rates

This study provides a rheological characterization of methyl cellulose solutions under high shear rates, showing their power-law behavior and temperature dependence.

Alcohol-sourced acetate impairs T cell function by promoting cortactin acetylation

This study shows alcohol-sourced acetate impairs T cell function by promoting cortactin acetylation, which disrupts actin binding and reduces migration and immune responses.

Adipose cells and tissues soften with lipid accumulation while in diabetes adipose tissue stiffens

This study investigates how adipocyte shape, size, and lipid accumulation affect mechanical properties of adipose tissue in health, obesity, and diabetes.

Viscoelastic properties of suspended cells measured with shear flow deformation cytometry

This paper introduces a microfluidic method to measure viscoelastic properties of suspended cells using shear flow deformation cytometry and demonstrates cell cycle-dependent rheological behavior.

Changes in blood cell deformability in Chorea-Acanthocytosis and effects of treatment with dasatinib or lithium

This study examines blood cell deformability in Chorea-Acanthocytosis patients and evaluates the effects of dasatinib or lithium treatment on red blood cell and leukocyte mechanics.

Mapping tumor spheroid mechanics in dependence of 3D microenvironment stiffness and degradability by Brillouin microscopy

This study uses Brillouin microscopy to map tumor spheroid mechanics in 3D hydrogels, showing that stiffness and degradability affect mechanical properties and cell invasion.

Label-free imaging flow cytometry: analysis and sorting of enzymatically dissociated tissues

The paper introduces methods to analyze and sort single cells from dissociated tissues using label-free imaging flow cytometry and deep learning, demonstrating enrichment of photoreceptor cells from retina for transplantation.

De novo identification of universal cell mechanics gene signatures

This study identifies a conserved gene module involved in cell mechanics regulation using a mechanomics approach combining mechanical characterization and machine learning analysis of transcriptomic data.

Quantitative imaging of Caenorhabditis elegans dauer larvae during cryptobiotic transition using optical diffraction tomography

This study uses optical diffraction tomography to quantify structural and material changes in Caenorhabditis elegans dauer larvae during desiccation.

Physical phenotype of blood cells is altered in COVID-19

The paper evaluates physical changes in blood cells of COVID-19 patients and finds persistent alterations in deformability and size of multiple cell types.

Quantitative coupling of cell volume and membrane tension during osmotic shocks

This study quantifies how cell volume and membrane tension change during osmotic shocks and shows they are coupled but not entirely independent.

Correlative all-optical quantification of mass density and mechanics of sub-cellular compartments with fluorescence specificity

This paper introduces FOB microscopy to measure sub-cellular density and mechanics with fluorescence specificity, revealing differences between nucleoplasm and cytoplasm.

Label-free composition determination for biomolecular condensates with an arbitrarily large number of components

This paper introduces a label-free method to measure the composition of biomolecular condensates with many components, showing it can capture complex dynamics and stoichiometry without perturbing the system.

HIF2α is a Direct Regulator of Neutrophil Motility

This study identifies HIF2α as a regulator of neutrophil motility through the PHD2-HIF2-RhoA axis, enhancing migration in hypoxic environments during inflammation.

The relative densities of cell cytoplasm, nucleoplasm, and nucleoli are robustly conserved during cell cycle and drug perturbations

The paper quantifies mass densities of cytoplasm, nucleoplasm, and nucleoli in human cells under cell cycle and drug perturbations, finding robust conservation of relative densities.

Using real-time fluorescence and deformability cytometry and deep learning to transfer molecular specificity to label-free sorting

Buckling of epithelium growing under spherical confinement

This study demonstrates that epithelial monolayers growing under spherical confinement can fold through buckling, showing forces and elastic properties drive the shape change.

Zebrafish spinal cord repair is accompanied by transient tissue stiffening

This study measures tissue stiffness changes in zebrafish spinal cord during repair, finding transient stiffening that correlates with cellular and structural changes.

High-throughput microfluidic characterization of erythrocyte shape and mechanical variability

This paper systematically characterizes red blood cell shape and dynamics in microfluidic channels, showing a distribution of states due to mechanical variability.

3D microenvironment stiffness regulates tumor spheroid growth and mechanics via p21 and ROCK

This study shows that 3D microenvironment stiffness affects tumor spheroid growth and mechanics through p21 and ROCK pathways.

Oncogenic signaling alters cell shape and mechanics to facilitate cell division under confinement.

This study shows that oncogenic Ras-ERK signaling changes cell mechanics and division behavior under confinement, aiding mitotic rounding and chromosome segregation.

Acute but not inherited demyelination in mouse models leads to brain tissue stiffness changes

This study shows that acute demyelination reduces brain tissue stiffness, while inherited demyelination does not, potentially affecting remyelination in mouse models.