BiophysicsbioRxiv
Quantitative determination of fluorescence labeling implemented in cell cultures
C. Schirripa Spagnolo, A. Moscardini, R. Amodeo…·S. Luin
The paper presents a method to quantitatively determine fluorescent labeling efficiency using ratiometric evaluation with two fluorophores in sequential reactions, validated through TrkA labeling in living cells.
DOI 10.1186/s12915-023-01685-0