Quantitative determination of fluorescence labeling implemented in cell cultures
C. Schirripa Spagnolo, A. Moscardini, R. Amodeo, F. Beltram, S. Luin
The paper presents a method to quantitatively determine fluorescent labeling efficiency using ratiometric evaluation with two fluorophores in sequential reactions, validated through TrkA labeling in living cells.
bioRxiv · Mar 27, 2023Biophysics