Charles Cole

The paper uses full-length cDNA sequencing to accurately characterize the human immune cell transcriptome, enabling HLA allele typing and adaptive immune receptor repertoire analysis.

R2C2 improves nanopore read accuracy for full-length single-cell cDNA sequencing, enabling more accurate isoform-level transcriptome analysis.

Tn5Prime is a 5' capture method for RNA-seq that enables accurate transcription start site identification and transcriptome quantification in single cells and bulk samples.

The paper uses long-read RNAseq to identify and quantify complex isoforms in individual B cells, revealing widespread transcriptional variation in surface receptors and the antibody heavy chain locus.