Absolute quantitation of individual SARS-CoV-2 RNA molecules: a new paradigm for infection dynamics and variant differences
J. Y. Lee, P. A. Wing, D. S. Gala, M. Noerenberg, A. I. Jarvelin, J. Titlow, X. Zhuang, N. Palmalux, L. Iselin, M. K. Thompson, R. M. Parton, A. Wainman, D. Agranoff, W. James, A. Castello, , I. Davis
The paper quantifies SARS-CoV-2 RNA molecules using smFISH, revealing genomic RNA stability, cell-to-cell replication variability, and slower replication kinetics for the B.1.1.7 variant compared to the Victoria strain.
bioRxiv · Jun 29, 2021Microbiology