I. Davis

Deep3DSIM: Super-resolution imaging of thick tissue using 3D structured illumination with adaptive optics

This paper introduces Deep3DSIM, an upright 3D-SIM system with adaptive optics for deep tissue imaging, achieving high-quality imaging up to 130 micrometers in Drosophila brain.

Sex-specific deubiquitylation drives immune-related neurodegeneration in Drosophila

This study shows that sex-specific deubiquitylation by Trabid in Drosophila influences neurodegeneration linked to immune responses and protein accumulation.

Dynamically regulated transcription factors are encoded by highly unstable mRNAs in the Drosophila larval brain

The paper measures RNA synthesis and decay rates in Drosophila larval brains, finding that transient transcription factor mRNAs are highly unstable and epigenetically silenced.

Mammalian glial protrusion transcriptomes predict localization of Drosophila glial transcripts required for synaptic plasticity

The paper predicts Drosophila glial transcripts involved in synaptic plasticity by analyzing mammalian glial transcriptomes and validates their localization and functional importance.

Systematic analysis of YFP gene traps reveals common discordance between mRNA and protein across the nervous system

The paper systematically analyzes YFP gene traps in Drosophila to show widespread discordance between mRNA and protein expression in the nervous system, revealing common post-transcriptional regulation.

Alphavirus infection triggers selective cytoplasmic translocation of nuclear RBPs with moonlighting antiviral roles

This study identifies nuclear RNA-binding proteins that are translocated to the cytoplasm during alphavirus infection and exhibit antiviral activity.

Absolute quantitation of individual SARS-CoV-2 RNA molecules: a new paradigm for infection dynamics and variant differences

The paper quantifies SARS-CoV-2 RNA molecules using smFISH, revealing genomic RNA stability, cell-to-cell replication variability, and slower replication kinetics for the B.1.1.7 variant compared to the Victoria strain.

Python-Microscope: A new open source Python library for the control of microscopes

Python-Microscope provides a scalable, open-source Python library for controlling complex custom microscopes and simulating microscope systems.

Microscope-Cockpit: Python-based bespoke microscopy for bio-medical science

The paper introduces Microscope-Cockpit, a Python-based GUI for controlling bespoke microscopes, enabling efficient specimen navigation and hardware integration through open-source tools.

Global analysis of protein-RNA interactions in SARS-CoV-2 infected cells reveals key regulators of infection

The paper identifies key host and viral RNA-binding proteins involved in SARS-CoV-2 infection and shows that drugs targeting these proteins can inhibit infection.

Wavefront-sensorless adaptive optics with a laser-free spinning disk confocal microscope

The paper introduces wavefront-sensorless adaptive optics for a laser-free spinning disk confocal microscope, improving signal, contrast, and resolution by correcting specimen-induced aberrations.

Democratising "Microscopi": a 3D printed automated XYZT fluorescence imaging system for teaching, outreach and fieldwork

The paper describes a low-cost, 3D-printed automated fluorescence imaging system for teaching and outreach, demonstrating its multimodal imaging capabilities and open-source design.

CryoSIM: super resolution 3D structured illumination cryogenic fluorescence microscopy for correlated ultra-structural imaging

The paper introduces cryoSIM, a super-resolution 3D fluorescence microscope for cryo-imaging that can be replicated with off-the-shelf components and open-source software.

Correlative cryo-structured illumination fluorescence microscopy and soft X-ray tomography elucidates reovirus intracellular release pathway

This study develops a correlative imaging platform combining cryo-SIM and SXT to study reovirus release from intracellular vesicles, revealing novel virus-induced structures.

IsoSense: frequency enhanced sensorless adaptive optics through structured illumination

IsoSense improves sensorless adaptive optics by using structured illumination to enhance spatial frequencies for reliable wavefront sensing in microscopy.