Single-cell analysis of a mutant library generated using CRISPR-guided deaminase
S. Jun, H. Lim, H. Chun, J. H. Lee, D. Bang
The paper develops a CRISPR-based platform combining deaminase and scRNA-seq to introduce and assess substitution mutations, enabling high-throughput analysis of mutation-associated transcriptional effects.
bioRxiv · Jan 21, 2020Bioengineering