Vector and cell-culture passaging of dengue clinical samples for virus isolation and amplification does not significantly change genome consensus or frequencies of intra-host viral variants
C. Fung, T. Li, S. Pollett, M. T. Alera, I.-K. Yoon, J. Hang, L. Macareo, A. Srikiatkhachorn, D. Ellison, A. L. Rothman, S. Fernandez, R. G. Jarman, I. Maljkovic Berry
This study shows that low-passage dengue virus isolates preserve the consensus sequence and intra-host variant frequencies of their clinical samples, making them suitable for genomic epidemiology.
bioRxiv · Aug 17, 2020 · PreprintEvolutionary Biology