D. Paredes-Sabja

Landscapes and bacterial signatures of mucosa-associated intestinal microbiota in Chilean and Spanish patients with inflammatory bowel disease

This study identifies bacterial biomarkers for distinguishing eubiosis from dysbiosis in IBD patients with high diagnostic accuracy.

Redistribution of the novel C. difficile spore-adherence receptor, E-cadherin, by TcdA and TcdB increases spore-binding to adherens junctions.

This study shows C. difficile spores bind to E-cadherin on intestinal epithelial cells, and toxins TcdA/TcdB increase spore adherence by disrupting adherens junctions.

Role of collagen-like protein BclA3 in the assembly of the exosporium layer of Clostridioides difficile spores.

This study investigates the role of BclA3 in assembling the exosporium layer of C. difficile spores, showing its necessity for hair-like projections and bump structures.

A ligated intestinal loop mouse model protocol to study the interactions of Clostridioides difficile spores with the intestinal mucosa during aging.

This paper describes a mouse model protocol to study C. difficile spore interactions with the intestinal mucosa during aging, finding higher spore internalization in elderly mice and reduced adherence with aging.

The Clostridioides difficile species problem: global phylogenomic analysis uncovers three ancient, toxigenic, genomospecies

This study identifies three ancient, toxigenic genomospecies within C. difficile using global phylogenomic analysis, reshaping its taxonomy and understanding of toxin gene evolution.

Clostridioides difficile spore-entry into intestinal epithelial cells contributes to recurrence of the disease

This study identifies mechanisms by which C. difficile spores enter intestinal epithelial cells, contributing to disease recurrence and suggesting therapeutic targets.

The cysteine-rich exosporium morphogenetic protein, CdeC, exhibits self-assembly properties that lead to organized inclusion bodies in Escherichia coli

This study investigates the self-assembly properties of CdeC, a cysteine-rich protein in C. difficile, showing it forms inclusion bodies in E. coli and suggests the C-terminal domain is critical for its organization.

Nasal immunization with the C-terminal domain of BclA3 induced specific IgG production and attenuated disease symptoms in mice infected with Clostridioides difficile spores

This study shows that the C-terminal domain of BclA3 induces IgG production and reduces CDI symptoms in mice, but spore-displayed antigen is less effective.

Comprehensive genome analyses of Sellimonas intestinalis, a potential biomarker of homeostasis gut recovery

This study characterizes Sellimonas intestinalis using genome sequencing and comparative analysis, revealing its potential as a biomarker for gut homeostasis recovery.

Characterization of exosporium layer variability of Clostridioides difficile spores in the epidemically relevant strain R20291.

The paper characterizes exosporium layer variability in C. difficile spores, showing differences in morphology and protein expression between thin and thick exosporium morphotypes.

cfrB,cfrC, and a potential new cfr-like gene in Clostridium difficile strains recovered across Latin America

The paper identifies and characterizes cfr-like genes in C. difficile strains from Latin America, showing diversity and functional activity of Cfr(C) and a potential new cfr-like gene.