Dose-Efficient Cryo-Electron Microscopy for Thick Samples using Tilt-Corrected Scanning Transmission Electron Microscopy, Demonstrated on Cells and Single Particles
Y. Yu, K. A. Spoth, M. Colletta, K. X. Nguyen, S. E. Zeltmann, X. S. Zhang, M. Paraan, M. Kopylov, C. Dubbeldam, D. Serwas, H. Siems, D. A. Muller, L. F. Kourkoutis
This paper introduces tilt-corrected bright-field scanning-TEM (tcBF-STEM) for cryo-EM, showing improved contrast and efficiency for thick samples, with a subnanometer resolution SPA map of a virus-like particle.
bioRxiv · Apr 22, 2024 · PreprintCell Biology