Cell BiologybioRxiv
Rapid sperm capture: High-throughput flagellar waveform analysis
M. T. Gallagher, G. Cupples, E. H. Ooi…·D. J. Smith
This paper introduces a fully-automated method for analyzing flagellar movement in sperm, revealing that CASA measures beat cross frequency instead of beat frequency and validating track centroid speed as a better indicator of progressive motility.