E. L. Decker

Differential GTP-dependent in-vitro polymerization of recombinant Physcomitrella FtsZ proteins

This study investigates the differential GTP-dependent polymerization of Physcomitrella FtsZ isoforms in vitro and their synergistic interactions.

Differential prolyl hydroxylation by six Physcomitrella prolyl-4 hydroxylases

This study identifies six prolyl-4 hydroxylases in mosses and characterizes their substrate specificity, structural features, and functional roles in proline hydroxylation and O-glycosylation.

Multifactorial analysis of terminator performance on heterologous gene expression in Physcomitrella

The paper characterizes Physcomitrella 3UTRs to identify effective terminators for gene expression, showing promoters have a greater impact than terminators.

Structural modelling of human complement FHR1 and two of its synthetic derivatives provides insight into their in-vivo functions

This study models the structure of FHR1 and its derivatives using AlphaFold2 and AlphaFold-Multimer to understand their interactions with complement components and their potential as therapeutic agents.

A Physcomitrella PIN protein acts in spermatogenesis and sporophyte retention

This study investigates the role of PpPINC in Physcomitrella, showing its importance in spermatogenesis and sporophyte retention through genetic and transcriptomic analyses.

Unexpected arabinosylation after humanization of plant protein N-glycosylation

This paper reports the unexpected arabinosylation observed during humanization of plant protein N-glycosylation, highlighting its implications for biopharmaceutical safety.

Process engineering of biopharmaceutical production in moss bioreactors via model-based description and evaluation of phytohormone impact

The paper investigates how phytohormone auxin enhances recombinant protein production in moss bioreactors and proposes a kinetic model for process optimization.

O-methylated N-glycans Distinguish Mosses from Vascular Plants

The paper identifies O-methylated N-glycans in mosses, distinguishing them from vascular plants and suggesting a closer phylogenetic relationship to green algae.

Autopolyploidization affects transcript patterns and gene targeting frequencies in Physcomitrella

This study examines how autopolyploidization affects gene expression and gene targeting efficiency in the moss Physcomitrella, finding altered transcript patterns and reduced gene targeting rates.

A synthetic protein as efficient multitarget regulator against complement over-activation

The paper designs a synthetic protein, MFHR13, that enhances complement regulation and shows improved therapeutic activity compared to existing treatments.

Medium optimization for biomass production of three peat moss (Sphagnum L.) species using fractional factorial design and response surface methodology

This study optimizes growth medium composition for three Sphagnum moss species using factorial design and response surface methodology, significantly increasing biomass yield in shake flasks and photobioreactors.

Stable Protein Sialylation in Physcomitrella

This study demonstrates stable N-glycan sialylation in genetically modified Physcomitrella patens by expressing mammalian enzymes and modifying galactosyltransferase genes.

Expression of a human cDNA in moss results in spliced mRNAs and fragmentary protein isoforms

The paper shows that expressing a human cDNA in moss leads to spliced mRNAs and non-functional protein isoforms, and presents a tool for codon optimization to improve protein yield.

Axenic in-vitro cultivation of nineteen peat-moss (Sphagnum L.) species as a resource for basic biology, biotechnology and paludiculture

The paper establishes axenic in-vitro cultures of 19 Sphagnum species to provide sustainable founder material for research and applications.