R. Reski

Differential GTP-dependent in-vitro polymerization of recombinant Physcomitrella FtsZ proteins

This study investigates the differential GTP-dependent polymerization of Physcomitrella FtsZ isoforms in vitro and their synergistic interactions.

An ancient role for the CYP73 gene family in t-cinnamic acid 4-hydroxylation, phenylpropanoid biosynthesis and embryophyte development

This study identifies the CYP73 gene family's ancient role in t-cinnamic acid 4-hydroxylation and embryophyte development through functional analysis in bryophytes.

Differential prolyl hydroxylation by six Physcomitrella prolyl-4 hydroxylases

This study identifies six prolyl-4 hydroxylases in mosses and characterizes their substrate specificity, structural features, and functional roles in proline hydroxylation and O-glycosylation.

Multifactorial analysis of terminator performance on heterologous gene expression in Physcomitrella

The paper characterizes Physcomitrella 3UTRs to identify effective terminators for gene expression, showing promoters have a greater impact than terminators.

Characterization of GAPDH expression related to biotic stress responses in Physcomitrella

The paper identifies and characterizes 17 GAPDH isoforms in Physcomitrella, showing differential regulation in response to SA and MeJA treatments and a potential role in defense against necrotrophic pathogens.

Long-Distance Electrical and Calcium Signals Evoked by Hydrogen Peroxide in Physcomitrella

This study shows hydrogen peroxide evokes long-distance electrical and calcium signals in Physcomitrella, with calcium and glutamate receptor ion channels playing key roles.

Structural modelling of human complement FHR1 and two of its synthetic derivatives provides insight into their in-vivo functions

This study models the structure of FHR1 and its derivatives using AlphaFold2 and AlphaFold-Multimer to understand their interactions with complement components and their potential as therapeutic agents.

A Physcomitrella PIN protein acts in spermatogenesis and sporophyte retention

This study investigates the role of PpPINC in Physcomitrella, showing its importance in spermatogenesis and sporophyte retention through genetic and transcriptomic analyses.

A deeply conserved protease, acylamino acid-releasing enzyme (AARE), acts in ageing in Physcomitrella and Arabidopsis

This study investigates the role of AARE proteases in plant ageing by analyzing mutants in Physcomitrella and Arabidopsis, linking AARE function to oxidized protein aggregation and lifespan.

Plasma membrane topography governs the three-dimensional dynamic localization of IgM B cell receptor clusters

This study uses lattice light sheet microscopy to show that plasma membrane topography influences the dynamic localization of IgM B cell receptor clusters in Ramos B cells.

Unexpected arabinosylation after humanization of plant protein N-glycosylation

This paper reports the unexpected arabinosylation observed during humanization of plant protein N-glycosylation, highlighting its implications for biopharmaceutical safety.

Process engineering of biopharmaceutical production in moss bioreactors via model-based description and evaluation of phytohormone impact

The paper investigates how phytohormone auxin enhances recombinant protein production in moss bioreactors and proposes a kinetic model for process optimization.

O-methylated N-glycans Distinguish Mosses from Vascular Plants

The paper identifies O-methylated N-glycans in mosses, distinguishing them from vascular plants and suggesting a closer phylogenetic relationship to green algae.

Autopolyploidization affects transcript patterns and gene targeting frequencies in Physcomitrella

This study examines how autopolyploidization affects gene expression and gene targeting efficiency in the moss Physcomitrella, finding altered transcript patterns and reduced gene targeting rates.

A synthetic protein as efficient multitarget regulator against complement over-activation

The paper designs a synthetic protein, MFHR13, that enhances complement regulation and shows improved therapeutic activity compared to existing treatments.

Medium optimization for biomass production of three peat moss (Sphagnum L.) species using fractional factorial design and response surface methodology

This study optimizes growth medium composition for three Sphagnum moss species using factorial design and response surface methodology, significantly increasing biomass yield in shake flasks and photobioreactors.

Stable Protein Sialylation in Physcomitrella

This study demonstrates stable N-glycan sialylation in genetically modified Physcomitrella patens by expressing mammalian enzymes and modifying galactosyltransferase genes.

Expression of a human cDNA in moss results in spliced mRNAs and fragmentary protein isoforms

The paper shows that expressing a human cDNA in moss leads to spliced mRNAs and non-functional protein isoforms, and presents a tool for codon optimization to improve protein yield.

Function of the HYDROXYCINNAMOYL-CoA:SHIKIMATE HYDROXYCINNAMOYL TRANSFERASE is evolutionarily conserved in embryophytes

This study shows that the HCT gene function is conserved in mosses and liverworts, supporting its ancient role in the phenylpropanoid pathway of embryophytes.

A NanoFE Simulation-based Surrogate Machine Learning Model to Predict Mechanical Functionality of Protein Networks from Live Confocal Imaging

The paper develops a machine learning model using live confocal imaging and nanoFE simulations to predict mechanical properties of FtsZ protein networks in moss chloroplasts.

Axenic in-vitro cultivation of nineteen peat-moss (Sphagnum L.) species as a resource for basic biology, biotechnology and paludiculture

The paper establishes axenic in-vitro cultures of 19 Sphagnum species to provide sustainable founder material for research and applications.

Spindle position dictates division site during asymmetric cell division in moss

This study shows that spindle position determines division site in moss during asymmetric cell division, challenging the notion that division planes are predetermined in plants.

Chloroplasts Require Glutathione Reductase to Balance Reactive Oxygen Species and Maintain Efficient Photosynthesis

This study shows that glutathione reductase is essential for maintaining a reducing stromal glutathione redox potential in chloroplasts, which supports efficient photosynthesis and plant growth.

Single-cell transcriptome analysis of Physcomitrella leaf cells during reprogramming using microcapillary manipulation

The paper introduces 1cell-DGE, a single-cell RNA-sequencing method using microcapillary manipulation to analyze gene expression in intact moss tissues, identifying differentially expressed genes during leaf cell reprogramming.