G. J. Schuetz

The paper uses live cell microscopy to show that monomeric agonist pMHCIIs can activate T-cells autonomously without requiring synaptic engagement or bystander pMHCIIs.

This study uses 3D-SMLM to measure the cleft size in immunological synapses between T-cells and lipid bilayers, finding distances of 18-31 nm.

This study evaluates strategies for site-specific decoration of DNA origami with functional proteins, finding that PNA-based conjugation best preserves protein function and stoichiometry.

The paper demonstrates that single pMHCs can robustly stimulate T-cells as isolated entities, identifying the minimal requirements for effective TCR-triggering.

The paper develops a method to quantify and correct for fluorophore blinking in PALM to improve cluster detection accuracy.

This paper introduces 2-CLASTA, a statistical method to detect biomolecular clusters in SMLM data, validated through simulations and experiments.