BiochemistrybioRxiv
Cell-surface tethered promiscuous biotinylators enable small-scale surface proteomics of human exosomes
L. L. Kirkemo, S. Elledge, J. Yang…·J. A. Wells
This paper introduces two methods for rapid, small-scale cell surface biotinylation using tethered enzymes, enabling surface proteome analysis of cells and exosomes with high coverage and quantification of 1,020 surface proteins.
DOI 10.7554/elife.73982