J. Kowalska

Trinucleotide mRNA cap analog N6-benzylated at the site of posttranscriptional m6Am mark facilitates mRNA purification and confers superior translational properties in vitro and in vivo

This paper introduces a modified mRNA cap analog, AvantCap, which improves mRNA purification and translational efficiency in vitro and in vivo.

Extracellular diadenosine tetraphosphate (Ap4A) is recognized by the plasma membrane purinoreceptor P2K1/DORN1 and closes stomata in Arabidopsis thaliana

The paper shows that Ap4A is recognized by P2K1/DORN1 in Arabidopsis, triggering stomatal closure and transcriptional changes, while Cp4C has opposing effects.

SARS-CoV-2 mRNA vaccine is re-adenylated in vivo, enhancing antigen production and immune response

This study shows that SARS-CoV-2 mRNA vaccines are re-adenylated in vivo, increasing antigen production and immune response in macrophages.

HIV-1 infection reduces NAD capping of host cell snRNA and snoRNA

This study shows HIV-1 infection reduces NAD capping of host snRNA and snoRNA, leading to increased HIV-1 infectivity by destabilizing RNA duplexes.

2'-O -methylation of the second transcribed nucleotide within mRNA 5' cap impacts protein production level in a cell specific manner and contributes to RNA immune evasion

This study shows that 2'-O-methylation of the second transcribed nucleotide in mRNA 5' cap affects protein production levels and contributes to RNA immune evasion in a cell-specific manner.

Functional and LC-MS/MS analysis of in vitro transcribed mRNAs carrying phosphorothioate or boranophosphate moieties reveal polyA tail modifications that prevent deadenylation without compromising protein expression

This study examines how phosphorothioate and boranophosphate modifications in mRNA polyA tails affect deadenylation resistance and protein expression.

The identity and methylation status of the first transcribed nucleotide in eukaryotic mRNA 5' cap modulates protein expression in living cells