A non-acidic method using hydroxyapatite and phosphohistidine monoclonal antibodies allows enrichment of phosphopeptides containing non-conventional phosphorylations for mass spectrometry analysis.
K. Adam, S. Fuhs, J. Meisenhelder, A. Aslanian, J. K. Diedrich, J. J. Moresco, J. La Clair, J. R. Yates, T. Hunter
This paper introduces a non-acidic method using hydroxyapatite and phosphohistidine antibodies to enrich non-conventional phosphopeptides for mass spectrometry analysis, detecting 425 unique phosphorylation sites.
bioRxiv · Jul 3, 2019 · PreprintBiochemistry