GenomicsbioRxivPreprint
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Efficient immune cell genome engineering with improved CRISPR editing tools
Waipan Chan, R. A. Gottschalk, Y. Yao…·R. N. Germain
Doxycycline-inducible dual-color Cas9, purified lentivirus, puromycin-selected nickase HDR, integrase-deficient lentiviral HDR delivery and an all-in-one CRISPRa vector enable editing of macrophage and Jurkat T cells.