Cell BiologybioRxiv
Fluorescent tagging of endogenous IRS2 with an auxin-dependent degron to assess dynamic intracellular localization and function
M. Jo, J.-S. Lee, M. W. Lero…·L. M. Shaw
This study uses CRISPR/Cas9 to fluorescently tag endogenous IRS2 with an auxin-dependent degron, revealing its dynamic nuclear-cytoplasmic shuttling and role in cancer stem cell function.
DOI 10.1016/j.jbc.2024.107796