Fluorescent tagging of endogenous IRS2 with an auxin-dependent degron to assess dynamic intracellular localization and function
M. Jo, J.-S. Lee, M. W. Lero, J. S. Morgan, L. M. Shaw
This study uses CRISPR/Cas9 to fluorescently tag endogenous IRS2 with an auxin-dependent degron, revealing its dynamic nuclear-cytoplasmic shuttling and role in cancer stem cell function.
bioRxiv · Dec 6, 2023Cell Biology