Highly Multiplexed 3D Profiling of Cell States and Immune Niches in Human Tumours
C. Yapp, A. J. Nirmal, F. Y. Zhou, A. Y. H. Wong, J. Tefft, Y. D. Lu, Z. Shang, Z. Maliga, P. Montero Llopis, G. F. Murphy, C. Lian, G. Danuser, S. Santagata, P. K. Sorger
This paper introduces 3D CyCIF, a method for high-plex 3D profiling of cell states and immune niches in human tumors, revealing detailed spatial interactions and cytokine niches previously unattainable in thin sections.
bioRxiv · Nov 10, 2023 · PreprintCancer Biology