C. Yapp

Highly Multiplexed 3D Profiling of Cell States and Immune Niches in Human Tumours

This paper introduces 3D CyCIF, a method for high-plex 3D profiling of cell states and immune niches in human tumors, revealing detailed spatial interactions and cytokine niches previously unattainable in thin sections.

2D and 3D multiplexed subcellular profiling of nuclear instability in human cancer

The paper shows nuclear rupture is common in cancer, especially glioblastoma, and links it to lamin proteins and immune signaling.

Multi-modal digital pathology for colorectal cancer diagnosis by high-plex immunofluorescence imaging and traditional histology of the same tissue section

The paper develops a multi-modal imaging platform combining H&E and high-plex immunofluorescence for colorectal cancer diagnosis, showing improved predictive models for progression-free survival.

Single Cell Spatial Analysis Reveals the Topology of Immunomodulatory Purinergic Signaling in Glioblastoma

The paper uses spatial single-cell analysis to show that CD73 and CD39 expression in glioblastoma correlates with poor clinical outcomes and immunosuppressive signaling.

The spatial landscape of progression and immunoediting in primary melanoma at single cell resolution

The paper uses spatial techniques to study immune evasion and immunoediting in primary melanoma, finding localized immunosuppressive environments and coexistence of invasion and regression.

Multiplexed 3D atlas of state transitions and immune interactions in colorectal cancer

This study uses multiplexed 3D imaging and machine learning to analyze colorectal cancer morphology and immune interactions, revealing molecular gradients and cell state transitions.

Age-dependent regulation of SARS-CoV-2 cell entry genes and cell death programs correlates with COVID-19 disease severity

The paper examines age-related changes in ACE2 expression and apoptosis regulation in lung tissue, linking these to differences in COVID-19 severity across age groups.

Highly multiplexed immunofluorescence images and single-cell data of immune markers in tonsil and lung cancer

The paper describes a dataset of multiplexed immunofluorescence images and single-cell immune marker data from human tonsil and lung cancer tissues, along with analysis methods and tools.

Combined inhibition of mTOR and PIKKs exploits replicative and checkpoint vulnerabilities to induce death of PI3K-activated triple-negative breast cancer cells

The paper shows that combining mTOR and PIKK inhibitors kills triple-negative breast cancer cells by exploiting replication and checkpoint vulnerabilities.

Highly multiplexed in situ protein imaging with signal amplification by Immuno-SABER

This paper introduces Immuno-SABER, a method for highly multiplexed protein imaging that overcomes limitations in sensitivity and throughput.

Highly multiplexed immunofluorescence imaging of human tissues and tumors using t-CyCIF and conventional optical microscopes

This paper introduces t-CyCIF, a method for highly multiplexed immunofluorescence imaging of FFPE tissues using conventional microscopes, and demonstrates its application in analyzing tumor antigens and immune markers.

Super-resolution microscopy of the β-carboxysome reveals a homogenous matrix

The paper uses super-resolution microscopy to study CcmM isoform localization in carboxysomes, finding both isoforms colocalize, revising the internal arrangement model.