G. Danuser

Anchorage-independent cell proliferation promoted by fascin's F-actin bundling

This study identifies fascin-1's role in promoting anchorage-independent proliferation through a novel signaling complex called PASS, which drives Rac1/PAK/JNK signaling in cancer cells.

Imaging of cellular dynamics in vitro and in situ: from a whole organism to sub-cellular imaging with self-driving, multi-scale microscopy

The paper introduces a self-driving, multi-resolution light-sheet microscope to simultaneously observe sub-cellular dynamics and whole organisms over extended periods, applying it to study development, cancer, and immune interactions in zebrafish.

Spatial proteomics of ER tubules reveals CLMN, an ER-actin tether at focal adhesions that promotes cell migration

The paper maps the human ER subdomain proteome, identifies CLMN as an ER-actin tether at focal adhesions, and shows it promotes cell migration by regulating actin dynamics and calcium signaling.

RhoA activation promotes glucose uptake to elevate proliferation in MAPK inhibitor resistant melanoma cells.

This study identifies RhoA activation as a driver of glucose uptake and proliferation in MAPK inhibitor-resistant melanoma cells through ROCK-mediated IRS1 and PI3K pathway activation.

Highly Multiplexed 3D Profiling of Cell States and Immune Niches in Human Tumours

This paper introduces 3D CyCIF, a method for high-plex 3D profiling of cell states and immune niches in human tumors, revealing detailed spatial interactions and cytokine niches previously unattainable in thin sections.

Endocytosis gated by emergent properties of membrane-clathrin interactions

This paper models clathrin-mediated endocytosis to show that the switch between productive and abortive CCPs is an emergent property of clathrin-membrane interactions and EAPs.

Keratin isoform shifts modulate motility signals during wound healing

The paper shows that keratin isoform shifts during wound healing modulate motility signals by activating myosin motors through isoform-specific interactions with vimentin shuttling kinases.

Compression-dependent microtubule reinforcement comprises a mechanostat which enables cells to navigate confined environments

The paper shows that microtubules form a mechanostat to coordinate contractility and nuclear positioning during confined cell migration.

In vivo profiling of site-specific human cancer cell states in zebrafish

The paper uses zebrafish xenografts and computer vision to study how Ewing Sarcoma cell morphology changes in different sites and under EWSR1-FLI1 expression variations.

Granger-causal inference of the lamellipodial actin regulator hierarchy by live cell imaging without perturbation

This study uses Granger-causal inference on live cell imaging data to reconstruct cause-effect relationships among actin regulators in lamellipodia without perturbation, identifying distinct activation zones and roles.

Blebs Promote Cell Survival by Assembling Oncogenic Signaling Hubs

This study shows blebs form signaling hubs that promote anoikis resistance in cancer cells by recruiting septins and activating ERK/PI3K pathways.

Talin-vinculin precomplex drives adhesion maturation by accelerated force transmission and vinculin recruitment

The paper shows that talin's precomplex with vinculin accelerates adhesion maturation by enhancing vinculin recruitment and force transmission.

u-track 3D: measuring and interrogating dense particle dynamics in three dimensions.

u-track 3D provides a software package for analyzing dense 3D particle dynamics in microscopy data by improving visualization, trajectory validation, and interactive exploration.

Proteolysis-free cell migration through crowded environments via mechanical worrying

The paper shows amoeboid melanoma cells migrate through crowded environments by mechanically degrading extracellular matrix via bleb-driven abrading, without proteolysis.

Interpretable deep learning of label-free live cell images uncovers functional hallmarks of highly-metastatic melanoma

This paper uses an adversarial auto-encoding deep learning model to classify metastatic efficiency in melanoma cells from label-free live cell images and identifies pseudopodial extensions and light scattering as functional hallmarks.

DASC, a sensitive classifier for measuring discrete early stages in clathrin-mediated endocytosis

The paper introduces DASC, a method to distinguish abortive clathrin coats from clathrin-coated pits in live-cell imaging, enabling sensitive analysis of CME progression in EAP knockdown conditions.

Direct Arp2/3-vinculin binding is essential for cell spreading, but only on compliant substrates and in 3D

The paper investigates the role of actin-binding proteins in cell spreading on 2D and 3D substrates, finding that Arp2/3-vinculin binding is critical for 3D spreading but not 2D.

Connectivity analysis of GEF/GTPase networks in living cells

The paper uses biosensors to analyze GEF-GTPase interactions in living cells and identifies functional relationships that regulate cell edge dynamics.

A unified role for membrane-cortex detachment during cell protrusion initiation

The paper shows that membrane-cortex detachment is essential for initiating both pressure-based and actin-based protrusions, challenging the Brownian ratchet model and unifying protrusion regulation.

Light-sheet microscopy with isotropic, sub-micron resolution and solvent-independent large-scale imaging

This paper introduces ctASLM, a light-sheet microscopy technique with sub-micron isotropic resolution and large-scale imaging, enabling automated cell and subcellular feature detection in cleared tissues.

An image-based assay to quantify changes in proliferation and viability upon drug treatment in 3D microenvironments

This paper introduces an image-based assay to measure drug effects on cell proliferation and viability in 3D microenvironments, showing 3D single cells are as effective as spheroids for drug screening.

Augmin-mediated amplification of long-lived spindle microtubules directs plus-ends to kinetochores

The paper shows that Augmin amplifies long-lived microtubules, directing their plus-ends to kinetochores during spindle assembly in human cells.

Cell morphological motif detector for high-resolution 3D microscopy images

The paper introduces a morphological motif detector for 3D microscopy images to analyze cell shape dynamics and signaling.

A non-canonical role for dynamin-1 in regulating early stages of clathrin-mediated endocytosis in non-neuronal cells

This study identifies a non-scission role for dynamin-1 in regulating early stages of clathrin-mediated endocytosis in non-neuronal cells through interactions with SNX9.

Diverse roles of guanine nucleotide exchange factors in regulating collective cell migration

The paper identifies GEFs regulating collective cell migration by screening human bronchial epithelial cells, showing RHOA GEFs influence intercellular communication and motility.

Decoupling global biases and local interactions between cell biological variables

The paper introduces DeBias, a method to separate global biases from local interactions in cell biology data, showing global bias contains mechanistic insights.