GeneticsbioRxivPreprint
Knock-In of a 25-Kilobase Pair BAC-Derived Donor Molecule by Traditional and CRISPR/Cas9-Stimulated Homologous Recombination
Tiffany Leidy-Davis, Kai Cheng, Leslie O. Goodwin…·David E. Bergstrom
The paper demonstrates the replacement of a 17-kbp mouse Bcl2l11 gene segment with a 25-kbp human BCL2L11 segment using traditional and CRISPR/Cas9 methods, achieving full humanization and functional validation.
DOI 10.1101/076612