M. E. Taga

This study evolves E. coli with a less-effective cobamide to identify genetic adaptations that improve growth, finding increased BtuB expression and BtuR overexpression enhance cobamide-dependent growth.

The paper investigates corrinoid production and dependence in soil bacteria to understand metabolic interactions, finding metabolic compatibility and conserved traits across genera.

The paper investigates virus-host dynamics in soil following rewetting, finding that a subset of viruses thrive and drive microbial lysis, contributing to CO2 efflux.

This study identifies a repressing and novel activating corrinoid riboswitch, enabling the engineering of synthetic riboswitches with robust gene induction.

This study shows that bacteriophage-mediated lysis releases more amino acids than secretion or mechanical lysis, supporting auxotroph growth.

The paper reports the draft genome sequence of Pedococcus sp. strain 5OH_020, isolated from California grassland soil, revealing its B12-dependent enzymes and potential as a novel species.

The paper investigates cobalamin riboswitches in Bacillus subtilis and finds they respond to multiple corrinoid cofactors, using structural conformation differences for regulation.

This study shows that precursor salvaging in engineered E. coli enables stable metabolite provisioning by allowing non-productive members to access salvaged cofactors while protecting salvagers from overexploitation.

The paper develops a chemically-defined medium that supports the growth of both Lactobacillus and Acetobacter bacteria, enabling the study of their metabolic interactions.

This paper identifies a novel cobamide remodeling enzyme, CbiR, in Akkermansia muciniphila that enables the bacterium to use diverse cobamides by modifying their structure.

The paper identifies unexpected diversity of CPR bacteria and nanoarchaea in grassland soil by analyzing particles less than 0.2 m, revealing new taxa and potential adaptations to aerobic conditions.

The paper investigates how different cobamides affect the function of methylmalonyl-CoA mutase and shows that non-cobalamin cobamides can also support its activity.

This study investigates cobamide metabolism in C. difficile, showing its ability to use diverse cobamides and bypass cobamide-dependent processes, with implications for disease treatment.

This study investigates how structural variations in cobamides affect the binding selectivity of methylmalonyl-CoA mutase, showing that in vitro binding correlates with in vivo cobamide dependence.

The paper uses comparative genomics to analyze cobamide biosynthesis and dependence in bacteria, finding that most bacteria use cobamides but few synthesize them de novo.