The His-tag as a decoy modulating preferred orientation in cryoEM
R. Bromberg, Y. Guo, D. A. Plymire, T. Emde, M. Puzio, D. Borek, Z. Otwinowski
The paper shows that His-tags affect particle orientation in cryoEM by modulating interactions with the water-air interface, suggesting their use for controlling preferred orientation patterns.
bioRxiv · Sep 22, 2020Biophysics