BiophysicsbioRxiv
The His-tag as a decoy modulating preferred orientation in cryoEM
R. Bromberg, Y. Guo, D. A. Plymire…·Z. Otwinowski
The paper shows that His-tags affect particle orientation in cryoEM by modulating interactions with the water-air interface, suggesting their use for controlling preferred orientation patterns.
DOI 10.3389/fmolb.2022.912072