Author

P. Diep

Listed by name: these papers carry no ORCID for this author, so different people with the same name may be mixed together.

Biology Disciplines

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Biochemistry· 4 Bioengineering· 2 Scientific Communication & Education· 1

Signature Assays, Organisms & Methods

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Approach:experimental assay· 7 Research Focus:protein folding & dynamics· 3 Research Focus:drug response / drug discovery· 2 Organism:E. coli· 2 Research Focus:benchmarking· 1 Assay:metabolomics· 1 Research Focus:protein design· 1 Assay:protein structure· 1

Frequent Co-Authors

R. Mahadevan· 7 shared A. F. Yakunin· 4 shared A. Yakunin· 2 shared B. Kell· 2 shared A. Boucinha· 1 shared A. Escobar· 1 shared A. Gnanapragasam· 1 shared A. Hilfinger· 1 shared

Activity by Year

2020-2022 · peak 3/yr

Co-Author Collaboration Network

10 top collaborators · 20 mutual co-author links

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7 shared papersBiochemistry

R. Mahadevan

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Author profile →

7 papers

Recent·Most cited

Quantifying metal ion specificity of the nickel-binding protein CcNikZ-II from Clostridium carboxidivorans in the presence of competing metal ions

P. Diep, B. Kell, A. Yakunin, A. Hilfinger, R. Mahadevan

The paper quantifies how the nickel-binding protein CcNikZ-II from Clostridium carboxidivorans binds different metal ions in the presence of competitors using a fluorescence quenching method and kinetic models.

bioRxiv · Oct 24, 2022 · PreprintBiochemistry

Ni(II) binding affinity and specificity of solute binding proteins: the importance of the double His motif and variable loop revealed by structural and mutational studies

P. Diep, P. J. Stogios, E. Evdokimova, A. Savchenko, R. Mahadevan, A. F. Yakunin

This paper investigates Ni(II) binding in solute binding proteins, showing the double His motif and variable loop are critical for affinity and specificity, with implications for biocatalytic applications.

bioRxiv · Oct 3, 2022 · PreprintBiochemistry

Engineered nickel bioaccumulation in Escherichia coli by NikABCDE transporter and metallothionein overexpression

P. Diep, L. Shen, J. A. Wiesner, N. Mykytczuk, V. Papangelakis, A. F. Yakunin, R. Mahadevan

This study engineered Escherichia coli to enhance nickel bioaccumulation using overexpressed NikABCDE transporter and metallothionein, achieving 7-fold improvement but with reduced cell viability.

bioRxiv · Aug 26, 2022Bioengineering

REVOLVER: a low-cost automated protein purifier based on parallel preparative gravity column workflows

P. Diep, J. L. C. Cardenas, A. F. Yakunin, A. P. McGuigan, R. Mahadevan

REVOLVER is a low-cost automated protein purification system that enables parallel processing with minimal human intervention, achieving results comparable to manual methods.

bioRxiv · Dec 12, 2021Biochemistry

Automation assisted anaerobic phenotyping for metabolic engineering

K. Raj, N. Venayak, P. Diep, S. A. Golla, A. F. Yakunin, R. Mahadevan

This paper develops an eco-friendly automation workflow for high-throughput anaerobic phenotyping to support metabolic engineering.

bioRxiv · May 3, 2021Bioengineering

Challenges in Undergraduate Synthetic Biology Training: Insights from a Canadian iGEM Student Perspective

P. Diep, A. Boucinha, B.-r. A. Yeung, B. Kell, X. Chen, D. Tsyplenkov, D. Serra, A. Escobar, A. Gnanapragasam, C. A. Emond, V. A. Sajtovich, R. Mahadevan, D. M. Kilkenny, G. Gini-Newman, M. Kaern, B. Ingalls

This study surveys Canadian undergraduate synthetic biology education through iGEM team experiences to provide insights and recommendations for teaching practices.

bioRxiv · Nov 4, 2020Scientific Communication & Education

A microplate screen to estimate metal-binding affinities of metalloproteins

P. Diep, R. Mahadevan, A. Yakunin

The paper introduces a high-throughput microITFQ-LTA method to screen metal-binding affinities of metalloproteins and validates it using two nickel-specific SBPs.

bioRxiv · Jun 16, 2020Biochemistry