A. Savchenko

A random mutagenesis screen enriched for missense mutations in bacterial effector proteins.

The paper presents a yeast-based method to identify informative missense mutations in bacterial effector proteins and validates it using three effectors from Legionella pneumophila.

Structural characterization of the Sel1-like repeat protein LceB from Legionella pneumophila

This study determines the structure of LceB, a core effector from Legionella pneumophila, revealing its conformational flexibility, self-association, and potential host protein-binding surface.

Environmental life cycle assessment of recombinant growth factor production for cultivated meat applications

This study uses life cycle assessment to evaluate the environmental impacts of producing recombinant growth factors for cultivated meat, finding significant global warming potentials for each factor.

Monomer and dimer structures of cytochrome bo3 ubiquinol oxidase from Escherichia coli

This study determines the monomeric and dimeric structures of E. coli cytochrome bo3 ubiquinol oxidase using cryo-EM, revealing dimerization through subunit interactions without major structural changes.

Functional diversification despite structural congruence in the HipBST toxin-antitoxin system of Legionella pneumophila

This study characterizes a conserved TA system in Legionella pneumophila, showing functional divergence from its E. coli counterpart despite structural similarity.

Ni(II) binding affinity and specificity of solute binding proteins: the importance of the double His motif and variable loop revealed by structural and mutational studies

This paper investigates Ni(II) binding in solute binding proteins, showing the double His motif and variable loop are critical for affinity and specificity, with implications for biocatalytic applications.

Thermophilic carboxylesterases from hydrothermal vents of the volcanic island of Ischia active on synthetic and biobased polymers and mycotoxins

This study identifies three novel thermotolerant carboxylesterases from hydrothermal vents that hydrolyze polyesters and mycotoxins, with potential for biotechnological applications.

Molecular mechanism of plasmid-borne resistance to sulfonamides

This study identifies the molecular mechanism of intrinsic sulfonamide resistance in Sul enzymes through structural and functional analyses, revealing a modified active site that confers high resistance without fitness cost.

Structural and molecular rationale for the diversification of resistance mediated by the Antibiotic_NAT family

The paper analyzes Antibiotic_NAT family enzymes to understand resistance diversification, finding environmental variants confer broad-spectrum resistance and likely ancestral relationships to clinical genes.

Structural characterization of Nonstructural protein 1 from SARS-CoV-2

The paper reports the crystal structure of SARS-CoV-2 Nsp1 and identifies structural features that inform modeling of related viral proteins.