Absolute Quantification of Viral Proteins from Pseudotyped Vesicular Stomatitis Virus (VSV-GP) using Ultra High-Performance Liquid Chromatography- Multiple Reaction Monitoring (UPLC-MRM)
R. Basu, R. Dambra, D. Jiang, S. A. Schätzlein, S. Nijyang, J. Ashour, A. Chiramel, A. Vigil, V. V. Papov
This paper develops a UPLC-MRM assay to absolutely quantify structural proteins of VSV-GP, including processed GP1/GP2 and full-length GPC, to assess viral processing and infectivity.
bioRxiv · Oct 9, 2023 · PreprintBiochemistry