BiophysicsbioRxivPreprint
Highly multiplexed spectral FLIM via physics informed data analysis
Mohamadreza Fazel, Reza Hoseini, Ayush Saurabh…·Steve Pressé
This paper introduces a Bayesian S-FLIM framework that learns spectra and lifetimes photon-by-photon, enabling efficient deconvolution of up to 9 fluorophore species with overlapping spectra.
DOI 10.1101/2025.08.04.668462