Cell BiologybioRxiv
A scalable strategy for high-throughput GFP tagging of endogenous human proteins
Manuel Leonetti, Sayaka Sekine, Daichi Kamiyama…·Bo Huang
This paper describes a scalable method for endogenous GFP tagging of human proteins using CRISPR-based electroporation, enabling proteomic and functional studies of native protein complexes.
DOI 10.1073/pnas.1606731113