BiochemistrybioRxiv
Influence of expression protocols on Gα biochemical activity: kinetics of plant and mammalian G protein cycles
T. E. Gookin, D. Chakravorty, S. M. Assmann
The paper compares expression protocols for Gα subunits, showing that a new purification method enhances enzymatic activity and reveals domain interactions affecting stability.
DOI 10.3389/fmolb.2025.1513660